Journal: Frontiers in Oncology

Article Title: Development and validation of an endoplasmic reticulum stress-related molecular prognostic model for breast cancer

doi: 10.3389/fonc.2023.1178595

Figure Lengend Snippet: Screening of ERS genes for independent prognostic factors and identification of ERScore. (A) Heat map of differentially expressed endoplasmic reticulum (ER) genes. (B) The variation curve of the coefficients of the variables with λ penalty. (C) Parameter plot of the penalty term with log(λ) values in the lower horizontal coordinates and degrees of freedom in the vertical coordinate. The model bias value was minimized when the variable was 16. (D) Multivariate Cox regression forest plot with FBXO6 , PMAIP1 , ERP27 , and CHAC1 as independent prognostic factors. (E) Classification of patients with breast cancer into high- and low-ERScore groups based on median ERScore values; patients in the high-ERScore group had a worse prognosis. *p<0.05, ***p<0.001.

Article Snippet: The membranes were blocked with 5% skim milk and incubated with primary antibodies for FBXO6(1:1000, 67697-1-Ig, Proteintech, China), PMAIP1 (1:1000, BM5042, Boster, China), ERP27 (1:1000, ab181172, Abcam, USA), CHAC1(1:1000, 15207-1-AP, Proteintech, China), or β-actin (1:2000, 3700, CST, USA) at 4°C overnight and secondary antibodies for Anti-rabbit IgG (1:4000, 7074, CST, USA) or Anti-mouse IgG(1:4000, 7076, CST, USA) at room temperature for 1 h. The bands were visualized using a Tanon 5500.

Techniques:

Journal: Frontiers in Oncology

Article Title: Development and validation of an endoplasmic reticulum stress-related molecular prognostic model for breast cancer

doi: 10.3389/fonc.2023.1178595

Figure Lengend Snippet: Differential expression of FBXO6 , PMAIP1 , ERP27 , and CHAC1 in breast and other cancer types. Expression of CHAC1 (A) , ERP27 (B) , FBXO6 (C) , and PMAIP1 (D) was elevated in breast cancer samples but results were inconsistent for other cancer types.

Article Snippet: The membranes were blocked with 5% skim milk and incubated with primary antibodies for FBXO6(1:1000, 67697-1-Ig, Proteintech, China), PMAIP1 (1:1000, BM5042, Boster, China), ERP27 (1:1000, ab181172, Abcam, USA), CHAC1(1:1000, 15207-1-AP, Proteintech, China), or β-actin (1:2000, 3700, CST, USA) at 4°C overnight and secondary antibodies for Anti-rabbit IgG (1:4000, 7074, CST, USA) or Anti-mouse IgG(1:4000, 7076, CST, USA) at room temperature for 1 h. The bands were visualized using a Tanon 5500.

Techniques: Quantitative Proteomics, Expressing

Journal: Frontiers in Oncology

Article Title: Development and validation of an endoplasmic reticulum stress-related molecular prognostic model for breast cancer

doi: 10.3389/fonc.2023.1178595

Figure Lengend Snippet: Distribution and validation of ER scores. (A) Correlation analysis of ERScore with FBXO6 , PMAIP1 , ERP27 , and CHAC1 , positive correlation with CHAC1 , and negative correlation with the remaining genes. (B) A risk factor heat map of ERScore displaying the relationship between ERScore and patient survival, as well as the abundance of FBXO6 , PMAIP1 , ERP27 , and CHAC1 in the ERScore group. (C) Time-dependent receiver operating characteristic curves of ERScore for the Cancer Genome Atlas-Breast Invasive Carcinoma (TCGA-BRCA) dataset.

Article Snippet: The membranes were blocked with 5% skim milk and incubated with primary antibodies for FBXO6(1:1000, 67697-1-Ig, Proteintech, China), PMAIP1 (1:1000, BM5042, Boster, China), ERP27 (1:1000, ab181172, Abcam, USA), CHAC1(1:1000, 15207-1-AP, Proteintech, China), or β-actin (1:2000, 3700, CST, USA) at 4°C overnight and secondary antibodies for Anti-rabbit IgG (1:4000, 7074, CST, USA) or Anti-mouse IgG(1:4000, 7076, CST, USA) at room temperature for 1 h. The bands were visualized using a Tanon 5500.

Techniques: Biomarker Discovery

Journal: Frontiers in Oncology

Article Title: Development and validation of an endoplasmic reticulum stress-related molecular prognostic model for breast cancer

doi: 10.3389/fonc.2023.1178595

Figure Lengend Snippet: Expression of four endoplasmic-reticulum-stress-related genes. Representative Western-blots show the protein expression of FBXO6, PMAIP1, ERP27, and CHAC1 in different cell lines. β-actin served as the normalization control.

Article Snippet: The membranes were blocked with 5% skim milk and incubated with primary antibodies for FBXO6(1:1000, 67697-1-Ig, Proteintech, China), PMAIP1 (1:1000, BM5042, Boster, China), ERP27 (1:1000, ab181172, Abcam, USA), CHAC1(1:1000, 15207-1-AP, Proteintech, China), or β-actin (1:2000, 3700, CST, USA) at 4°C overnight and secondary antibodies for Anti-rabbit IgG (1:4000, 7074, CST, USA) or Anti-mouse IgG(1:4000, 7076, CST, USA) at room temperature for 1 h. The bands were visualized using a Tanon 5500.

Techniques: Expressing, Western Blot, Control